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Capillary Electrophoresis

Equipment:

PA 800 Plus Pharmaceutical Analysis System (Sciex) equipped with UV absorbance and diode array detectors.

Method:

Capillary electrophoresis (CE) is a family of electrokinetic separation methods, all of them applicable for the analysis of proteins and peptides. CE combines the advantages of conventional gel electrophoresis and high-performance liquid chromatography to separate solutes according to their ability to migrate in an electric field inside a fused silica capillary. It employs narrow-bore (20-200 µm i.d.) capillaries to perform separations of both large and small molecules. The instrumental configuration for CE is relatively simple. All that is required is a fused-silica capillary with an optical viewing window, a controllable high voltage power supply, two electrode assemblies, two buffer reservoirs, and a detector. Method-inherent advantages are highly efficient separations, on-line detection, precise quantification, and automation. Separation modes available in our lab are capillary zone electrophoresis (CZE) and capillary gel electrophoresis (CGE).

Sample Preparation:

  • Samples can either be dissolved in water (most suitable) or in buffers with known composition, pH value etc. 
  • Samples can also be lyophilized.

Provided Services:

  • Quality control of purified proteins (e. g. Monoclonal Antibodies) or peptides with focus on
    • Purity/Heterogeneity
    • posttranslational modifications (e.g. glycosylation, phosphorylation, ...)
  • Quality control of Plasmids and Nucleic Acid Therapeutics
  • Analysis of viruses, subviral particles and virus complexes

If method development or other CE modes (MEKC, CIEF) are required, please contact us via “e-mail”.

Fees for CE:

For further details and price, please contact us via “e-mail

Sample submission form. Download "here".